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KMID : 0861020150300010085
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Korea Journal of Herbology
2015 Volume.30 No. 1 p.85 ~ p.93
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The Effects of bilobalide Extracted from Ginkgonis Folium on Inflammation
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Jung Je-Ryong
Kill Ki-Jung
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Abstract
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Objectives : Bilobalide (BIL) is a predominant sesquiterpene trilactone constituent that accounts for a partial portion of the standardized Ginkgonis Folium extract, which has been widely used to treat a variety of neurological disorders involving cerebral ischemia and neurodegeneration. In this study, it was tested whether BIL exhibits anti-inflammatory activities on inflammation response, or not.
Methods : To elucidate the molecular mechanisms of BIL on pharmacological and biochemical actions in inflammation, we examined the effect of BIL on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages. The investigation was focused on how BIL affect on inflammation-related mediators including various signals such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase(iNOS), cyclooxygenase-2(COX-2), interleukin-6(IL-6), tumor necrosis factor-α (TNF-α), mitogen-activated protein kinases(MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-кB) in LPS-stimulated RAW 264.7 cells.
Results : We found that BIL inhibited LPS-induced NO, PGE2, IL-6 and TNF-α productions as well as the expressions of iNOS and COX-2. Furthermore, BIL suppressed the LPS-induced phosphorylation for MAPK activation.
Conclusions : These results suggest that BIL has inhibitory effects on LPS-induced PGE2, NO, IL-6 and TNF-α production, as well as the expressions of iNOS and COX-2 in the murine macrophage. It seems that these inhibitory effects occur by blocking the phosphorylation of MAPKs for activation. Then, BIL suppressed the activation of nuclear factor NF-кB in nucleus. These observations suggest that BIL has anti-inflammatory effect by inhibiting.
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KEYWORD
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Bilobalide, RAW 264.7 cells, Lipopolysaccharide, Inflammation
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